Cdk13^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cdk13^{tm1d(EUCOMM)Hmgu}
• Name: cyclin dependent kinase 13; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6451101
• Gene: Cdk13 Location: Chr13:17885309-17979960 bp, - strand Genetic Position: Chr13, 6.05 cM, cytoband A3.1
• Alliance: Cdk13^{tm1d(EUCOMM)Hmgu} page

Mutation origin

• Mutant Cell Line: HEPD0683_5_G02
• Germline Transmission: Earliest citation of germline transmission: J:291583
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 17939281 of Chromosome 13 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 17941612. The critical exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exons 3 and 4. Western blot analysis confirmed absence of protein expression in the brain. (J:291583)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk13 Mutation: 47 strains or lines available

References

• Original: J:291583 Novakova M, et al., Mouse Model of Congenital Heart Defects, Dysmorphic Facial Features and Intellectual Developmental Disorders as a Result of Non-functional CDK13. Front Cell Dev Biol. 2019;7:155
• All: 3 reference(s)