Cdk13tm1d(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Cdk13tm1d(EUCOMM)Hmgu |
Name: |
cyclin dependent kinase 13; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:6451101 |
Gene: |
Cdk13 Location: Chr13:17885309-17979960 bp, - strand Genetic Position: Chr13, 6.05 cM, cytoband A3.1
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Alliance: |
Cdk13tm1d(EUCOMM)Hmgu page
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Mutant Cell Line: |
HEPD0683_5_G02 |
Germline Transmission: |
Earliest citation of germline transmission:
J:291583
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 17939281 of Chromosome 13 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 17941612. The critical exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exons 3 and 4. Western blot analysis confirmed absence of protein expression in the brain.
(J:291583)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Cdk13 Mutation: |
44 strains or lines available
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Original: |
J:291583 Novakova M, et al., Mouse Model of Congenital Heart Defects, Dysmorphic Facial Features and Intellectual Developmental Disorders as a Result of Non-functional CDK13. Front Cell Dev Biol. 2019;7:155 |
All: |
3 reference(s) |
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