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Cdk13tm1d(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Cdk13tm1d(EUCOMM)Hmgu
Name: cyclin dependent kinase 13; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:6451101
Gene: Cdk13  Location: Chr13:17885309-17979960 bp, - strand  Genetic Position: Chr13, 6.05 cM, cytoband A3.1
Alliance: Cdk13tm1d(EUCOMM)Hmgu page
Mutation
origin
Mutant Cell Line:  HEPD0683_5_G02
Germline Transmission:  Earliest citation of germline transmission: J:291583
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 17939281 of Chromosome 13 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 17941612. The critical exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exons 3 and 4. Western blot analysis confirmed absence of protein expression in the brain. (J:291583)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 8 assay results
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cdk13 Mutation:  46 strains or lines available
References
Original:  J:291583 Novakova M, et al., Mouse Model of Congenital Heart Defects, Dysmorphic Facial Features and Intellectual Developmental Disorders as a Result of Non-functional CDK13. Front Cell Dev Biol. 2019;7:155
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory