Oprm1^{tm1.1(cre/GFP)Rpa}
Targeted Allele Detail

Summary

• Symbol: Oprm1^{tm1.1(cre/GFP)Rpa}
• Name: opioid receptor, mu 1; targeted mutation 1.1, Richard D Palmiter
• MGI ID: MGI:6451472
• Synonyms: Oprm1^Cre, Oprm1^Cre:GFP
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^{tm1.1(cre/GFP)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325015
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutations: Insertion, Intragenic deletion
• Oprm1^{tm1.1(cre/GFP)Rpa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Oprm1 (MGI:97441)

• Mutation details: A cassette encoding Cre:GFP (a Myc-tag and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 5' of the initiation codon in the first coding exon of the Oprm1 gene replacing the exon 1 via homologous recombination. The neomycin cassette was excised of through crosses with animals broadly expressing Flp recombinase. (J:314358, J:325015)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Oprm1 (mouse) Summary of all recombinase alleles driven by Oprm1. Comparative matrix view of recombinase activities Alliance mouse Oprm1 gene page

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 54 strains or lines available

References

• Original: J:325015 Liu S, et al., Divergent brainstem opioidergic pathways that coordinate breathing with pain and emotions. Neuron. 2022 Mar 2;110(5):857-873.e9
• All: 7 reference(s)