Ptger3^{tm1.1(cre/GFP)Rpa}
Targeted Allele Detail

Summary

• Symbol: Ptger3^{tm1.1(cre/GFP)Rpa}
• Name: prostaglandin E receptor 3 (subtype EP3); targeted mutation 1.1, Richard D Palmiter
• MGI ID: MGI:6451475
• Gene: Ptger3 Location: Chr3:157272459-157350392 bp, + strand Genetic Position: Chr3, 81.32 cM
• Alliance: Ptger3^{tm1.1(cre/GFP)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331457
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Ptger3^{tm1.1(cre/GFP)Rpa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ptger3 (MGI:97795)

• Mutation details: A cassette encoding Cre:GFP (a Myc-tag and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 5' of the initiation codon in the first coding exon of the Ptger3 gene via homologous recombination. The neomycin cassette was excised of through crosses with animals broadly expressing Flp recombinase. (J:331457)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ptger3 (mouse) Summary of all recombinase alleles driven by Ptger3. Comparative matrix view of recombinase activities Alliance mouse Ptger3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ptger3 Mutation: 19 strains or lines available

References

• Original: J:331457 Pauli JL, et al., Molecular and anatomical characterization of parabrachial neurons and their axonal projections. Elife. 2022 Nov 1;11
• All: 2 reference(s)