Gt(ROSA)26Sor^{tm2(CAG-COP4*C128S*D156A*T159C,-tdTomato)Gfng}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-COP4*C128S*D156A*T159C,-tdTomato)Gfng}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Guoping Feng
• MGI ID: MGI:6451493
• Synonyms: R26-LSL-SOUL-P2A-tdT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-COP4*C128S*D156A*T159C,-tdTomato)Gfng} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292780
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct is constructed by first using the Ai9 targeting vector modified to replace the tdTomato fragment with a SOUL-P2A-tdTomato cassette - from 5' to 3' - a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a FRT site, a loxP-flanked STOP cassette, the SOUL sequence (described below), a viral 2A oligopeptide, a tdTomato sequence, a woodchuck hepatitis virus post-transcriptional regulatory element, a bovine growth hormone polyA sequence (BGHpA) and an AttB/AttP-flanked PGK-frt-Neo-polyA cassette. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The step-function opsin with ultra-high light sensitivity gene (SOUL; COP4*C128S*D156A*T159C) cDNA sequence encodes a channelrhodopsin-2 (ChR2; COP4 from the green alga Chlamydomonas reinhardtii) modified to have three amino acid substitutions that result in step function-like properties with extended channel open state (C128S/D156A) and enhanced photocurrent amplitudes (T159C). (J:292780)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:292780 Gong X, et al., An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques. Neuron. 2020 Jul 8;107(1):38-51.e8
• All: 3 reference(s)