Pcif1^em2Rspi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pcif1^em2Rspi
• Name: phosphorylated CTD interacting factor 1; endonuclease-mediated mutation 2, Ramesh S Pillai
• MGI ID: MGI:6452631
• Synonyms: PCIF1-3xFLAG, Pcif1^em2Embrp
• Gene: Pcif1 Location: Chr2:164721288-164733360 bp, + strand Genetic Position: Chr2, 85.27 cM
• Alliance: Pcif1^em2Rspi page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1/J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted a four-amino acid flexible linker (SGGG) followed by a triple FLAG tag (MDYKDHDGDYKDHDIDYKDDD) upstream of the translation stop codon. (J:293136)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcif1 Mutation: 44 strains or lines available

References

• Original: J:293136 Pandey RR, et al., The Mammalian Cap-Specific m(6)Am RNA Methyltransferase PCIF1 Regulates Transcript Levels in Mouse Tissues. Cell Rep. 2020 Aug 18;32(7):108038
• All: 1 reference(s)