Tmprss12^em4Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmprss12^em4Osb
• Name: transmembrane (C-terminal) protease, serine 12; endonuclease-mediated mutation 4, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6455010
• Synonyms: Tmprss12^-8,-7871+5, Tmprss12^del
• Gene: Tmprss12 Location: Chr15:100178743-100190943 bp, + strand Genetic Position: Chr15, 56.24 cM
• Alliance: Tmprss12^em4Osb page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA/2J)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR-targeting of exon 2 deleted 8 bp within the intron with the indel -7871 bp, +5 bp between intron 2 and exon 5 to produce a frameshift mutation with a premature stop codon at the 70th amino acid. Western blot analysis confirmed the absence of protein expression in testicular giant cells and spermatozoa. (J:293874)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tmprss12 Mutation: 30 strains or lines available

References

• Original: J:293874 Larasati T, et al., Tmprss12 is required for sperm motility and uterotubal junction migration in mice. Biol Reprod. 2020 Aug 4;103(2):254-263
• All: 1 reference(s)