Coq8b^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Coq8b^{tm1c(EUCOMM)Hmgu}
• Name: coenzyme Q8B; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6455494
• Synonyms: Adck4^flox
• Gene: Coq8b Location: Chr7:26932448-26957375 bp, + strand Genetic Position: Chr7, 15.85 cM, cytoband A3
• Alliance: Coq8b^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294296
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26938245 of Chromosome 7 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 26939943. Exons 5 and 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:294296)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Coq8b Mutation: 30 strains or lines available

References

• Original: J:294296 Widmeier E, et al., ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment. J Am Soc Nephrol. 2020 Jun;31(6):1191-1211
• All: 1 reference(s)