Ralgapa1^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ralgapa1^{tm2c(KOMP)Wtsi}
• Name: Ral GTPase activating protein, alpha subunit 1; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:6456655
• Gene: Ralgapa1 Location: Chr12:55649681-55867952 bp, - strand Genetic Position: Chr12, 24.06 cM
• Alliance: Ralgapa1^{tm2c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0573_2_A12
• Germline Transmission: Earliest citation of germline transmission: J:287672
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55832731 of Chromosome 12 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 55833576. The critical exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287672)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ralgapa1 Mutation: 143 strains or lines available

References

• Original: J:287672 Chen Q, et al., Targeting RalGAPalpha1 in skeletal muscle to simultaneously improve postprandial glucose and lipid control. Sci Adv. 2019 Apr;5(4):eaav4116
• All: 1 reference(s)