Garem2^tm1.2Hkon
Targeted Allele Detail

Summary

• Symbol: Garem2^tm1.2Hkon
• Name: GRB2 associated regulator of MAPK1 subtype 2; targeted mutation 1.2, Hiroaki Konishi
• MGI ID: MGI:6456657
• Gene: Garem2 Location: Chr5:30310194-30323378 bp, + strand Genetic Position: Chr5, 15.92 cM
• Alliance: Garem2^tm1.2Hkon page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287781
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT flanked neomycin resistance cassette with a 3' loxP site were inserted upstream of exon 4. Another loxP site was inserted downstream of exon4. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 4. Western blot analysis confirmed the absence of protein expression in the whole brain. (J:287781)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Garem2 Mutation: 30 strains or lines available

References

• Original: J:287781 Nishino T, et al., Behavioral analysis in mice deficient for GAREM2 (Grb2-associated regulator of Erk/MAPK subtype2) that is a subtype of highly expressing in the brain. Mol Brain. 2019 Nov 12;12(1):94
• All: 1 reference(s)