Chd3^tm1.1Cya
Targeted Allele Detail

Summary

• Symbol: Chd3^tm1.1Cya
• Name: chromodomain helicase DNA binding protein 3; targeted mutation 1.1, Cyagen Biosciences
• MGI ID: MGI:6456882
• Synonyms: Chd3-flox, Chd3^tm1.1Cgen
• Gene: Chd3 Location: Chr11:69234099-69260232 bp, - strand Genetic Position: Chr11, 42.53 cM
• Alliance: Chd3^tm1.1Cya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294633
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 12 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 20. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exons 13-20 floxed. (J:294633)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Chd3^tm1.1Cya/Chd3^tm1.1Cya Edil3^Tg(Sox2-cre)1Amc/0	involves: C57BL/6 * C57BL/6J * CBA
cn2	Chd3^tm1.1Cya/Chd3^tm1.1Cya Tg(Tek-cre)1Ywa/0	involves: C57BL/6 * C57BL/6J * SJL
cn3	Chd3^tm1.1Cya/Chd3^tm1.1Cya Chd4^tm1.1Kge/Chd4^tm1.1Kge Tg(Tek-cre)1Ywa/0	involves: C57BL/6 * C57BL/6J * SJL

Phenotypes:

Affected Systems	cn1	cn2	cn3
show or hide all annotated terms
cardiovascular system		√	√
cardiovascular system phenotype		N
abnormal blood vessel morphology		√	√
hemorrhage			√
mortality/aging	√	N	√
mortality/aging		N
embryonic lethality, complete penetrance			√
embryonic lethality, incomplete penetrance	√
reproductive system	N
reproductive system phenotype	N

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chd3 Mutation: 103 strains or lines available

References

• Original: J:294633 Xie J, et al., The chromatin-remodeling enzyme CHD3 plays a role in embryonic viability but is dispensable for early vascular development. PLoS One. 2020;15(7):e0235799
• All: 2 reference(s)