Hmgcs2^tm1.1Yil
Targeted Allele Detail

Summary

• Symbol: Hmgcs2^tm1.1Yil
• Name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2; targeted mutation 1.1, Omer YIlmaz
• MGI ID: MGI:6457108
• Synonyms: Hmgcs2^loxp
• Gene: Hmgcs2 Location: Chr3:98187751-98218054 bp, + strand Genetic Position: Chr3, 42.74 cM
• Alliance: Hmgcs2^tm1.1Yil page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280699
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted upstream of exon 2 (Build GRCm38). The L1L2_Bact_P cassette was composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), an IRES enhancer, the beta-galactosidase gene (lacZ), an SV40 polyadenylation signal (SV40pA), a loxP site, a human beta-actin promoter (hbactP), the neomycin-resistance gene (neo), a second SV40 polyadenylation signal (SV40pA), a second FRT site, and a second loxP site. A third loxP site was inserted downstream of exon 2. KOMP vector PG00052_Z_4_A06 was used. Flp-mediated recombination removed the FRT-flanked neo:loxP:lacZ cassette leaving exon 2 floxed. (J:280699)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hmgcs2 Mutation: 33 strains or lines available

References

• Original: J:280699 Cheng CW, et al., Ketone Body Signaling Mediates Intestinal Stem Cell Homeostasis and Adaptation to Diet. Cell. 2019 Aug 22;178(5):1115-1131.e15
• All: 3 reference(s)