Tg(Thy1/THY1)T12Gsv
Transgene Detail

Summary

• Symbol: Tg(Thy1/THY1)T12Gsv
• Name: transgene insertion T12, Frank Grosveld
• MGI ID: MGI:6458466
• Synonyms: T12
• Transgene: Tg(Thy1/THY1)T12Gsv Location: unknown
• Alliance: Tg(Thy1/THY1)T12Gsv page

Transgene origin

• Strain of Origin: (CBA x C57BL/10)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1/THY1)T12Gsv expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  mouse

  Thy1 (MGI:98747)

  hybrid of mouse and human sequences

  human

  THY1 (7070)

  Thy1	(MGI:98747)

  hybrid of mouse and human sequences

• Mutation details: This transgene consists of a hybrid Thy1 gene in which a 5.7 kb EcoRI-ApaI mouse Thy1^a (Thy1.1) gene fragment containing the first 4 exons (including exons 1a and 1b) and part of exon 4 are fused to a 3.4 kb ApaI-EcoRI human THY1 DNA sequence containing the remaining noncoding part of exon 4 plus 3' human flanking sequence. Four lines were generated; T6, T9, T11, and T12. The T12 line contains multiple copies of the transgene and expresses about 6-10x the normal level of Thy1 in the brain. The mature protein resulting from this transgene is completely Thy1^a. High levels of hybrid transgene expression is seen in glomerular podocytes. (J:133943)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:133943 Kollias G, et al., Ectopic expression of Thy-1 in the kidneys of transgenic mice induces functional and proliferative abnormalities. Cell. 1987 Oct 9;51(1):21-31
• All: 1 reference(s)