Wbp1l^{tm2c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Wbp1l^{tm2c(EUCOMM)Hmgu}
• Name: WW domain binding protein 1 like; targeted mutation 2c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6458551
• Synonyms: Wbp1l^fl
• Gene: Wbp1l Location: Chr19:46587545-46645828 bp, + strand Genetic Position: Chr19, 38.97 cM
• Alliance: Wbp1l^{tm2c(EUCOMM)Hmgu} page
• IMPC: Wbp1l gene page

Mutation origin

• Mutant Cell Line: HEPD0797_6_B06
• Germline Transmission: Earliest citation of germline transmission: J:284781
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 46642038 of Chromosome 19 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 46643588. The critical exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:284781)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
cn1	Wbp1l^tm2c(EUCOMM)Hmgu/Wbp1l^tm2c(EUCOMM)Hmgu Gt(ROSA)26Sor^tm1(cre/ERT2)Tyj/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * C57BL/6N * SJL

Phenotypes:

Affected Systems	cn1
show or hide all annotated terms
hematopoietic system	√
abnormal bone marrow cell physiology	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wbp1l Mutation: 25 strains or lines available

References

• Original: J:284781 Borna S, et al., Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis. J Cell Mol Med. 2020 Jan;24(2):1980-1992
• All: 2 reference(s)