Dguok^tm1.1Anka
Targeted Allele Detail

Summary

• Symbol: Dguok^tm1.1Anka
• Name: deoxyguanosine kinase; targeted mutation 1.1, Anna Karlsson
• MGI ID: MGI:6460395
• Synonyms: Dguok^-
• Gene: Dguok Location: Chr6:83457199-83483887 bp, - strand Genetic Position: Chr6, 35.94 cM
• Alliance: Dguok^tm1.1Anka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294620
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked PGK-neomycin selection cassette with a 3' loxP site was inserted downstream of exon 2. Cre-mediated recombination simultaneously removed exon 2 and the selection cassette. Real-time PCR analysis confirmed the absence of mRNA expression in liver tissue from homozygous mutant mice. (J:294620)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dguok Mutation: 12 strains or lines available

References

• Original: J:294620 Zhou X, et al., Severe mtDNA depletion and dependency on catabolic lipid metabolism in DGUOK knockout mice. Hum Mol Genet. 2019 Sep 1;28(17):2874-2884
• All: 2 reference(s)