Tmem95^em1Beal
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmem95^em1Beal
• Name: transmembrane protein 95; endonuclease-mediated mutation 1, Pablo Bermejo-Alvarez
• MGI ID: MGI:6460450
• Gene: Tmem95 Location: Chr11:69766883-69768879 bp, - strand Genetic Position: Chr11, 42.91 cM
• Alliance: Tmem95^em1Beal page

Mutation origin

• Strain of Origin: (CBA x C57BL/6)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Intragenic deletion, Nucleotide substitutions
• Mutation details: CRISPR/cas9 mediated recombination created a 4bp substitution (ACTT to GAAG) followed by a 10 bp deletion (CGGCTTCGTC) in exon 1 predicted to result in a truncated transcript. Immunohistochemical analysis confirmed the absence of protein expression in sperm from homozygous mice. (J:289090)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem95 Mutation: 9 strains or lines available

References

• Original: J:289090 Lamas-Toranzo I, et al., TMEM95 is a sperm membrane protein essential for mammalian fertilization. Elife. 2020 Jun 2;9:e53913
• All: 1 reference(s)