Rsph9^em1Jwji
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rsph9^em1Jwji
• Name: radial spoke head 9 homolog (Chlamydomonas); endonuclease-mediated mutation 1, Jianwei Jiao
• MGI ID: MGI:6466630
• Synonyms: Rsph9^-
• Gene: Rsph9 Location: Chr17:46432961-46455142 bp, - strand Genetic Position: Chr17, 22.87 cM, cytoband C
• Alliance: Rsph9^em1Jwji page

Mutation origin

• Strain of Origin: (C57BL/6 x 129S/SvEv)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An 8 bp deletion was generated by CRISPR/Cas9 technology, producing a premature stop codon at the end of exon 1 that significantly truncates the encoded protein. The deletion was confirmed by immunofluorescence staining in brain ependymal cilia and tracheal cilia from homozygous mutant mice. (J:294184)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rsph9 Mutation: 18 strains or lines available

References

• Original: J:294184 Zou W, et al., Loss of Rsph9 causes neonatal hydrocephalus with abnormal development of motile cilia in mice. Sci Rep. 2020 Jul 24;10(1):12435
• All: 1 reference(s)