Slc6a3^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc6a3^{tm1c(KOMP)Wtsi}
• Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6467307
• Synonyms: Dat^fl
• Gene: Slc6a3 Location: Chr13:73684866-73726791 bp, + strand Genetic Position: Chr13, 40.1 cM
• Alliance: Slc6a3^{tm1c(KOMP)Wtsi} page
• IMPC: Slc6a3 gene page

Mutation origin

• Mutant Cell Line: EPD0304_3_A04
• Germline Transmission: Earliest citation of germline transmission: J:280705
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 73704721 of Chromosome 13 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 73705541. The critical exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:280705)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc6a3 Mutation: 66 strains or lines available

References

• Original: J:280705 Wu J, et al., Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning. Cell Rep. 2019 Jul 30;28(5):1167-1181.e7
• All: 1 reference(s)