Eef1d^tm1.2Mkma
Targeted Allele Detail

Summary

• Symbol: Eef1d^tm1.2Mkma
• Name: eukaryotic translation elongation factor 1 delta; targeted mutation 1.2, Masayuki Matsushita
• MGI ID: MGI:6467473
• Synonyms: eEF1BdeltaL-KO
• Gene: Eef1d Location: Chr15:75766643-75781425 bp, - strand Genetic Position: Chr15, 35.05 cM, cytoband E1
• Alliance: Eef1d^tm1.2Mkma page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288930
• Parent Cell Line: B6-3i/HK3i (ES Cell)
• Strain of Origin: C57BL/6NJcl

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: LoxP sites and an frt-flanked neomycin selection cassette were inserted to flank exon 3. The frt-flanked neomycin selection cassette and exon 3 were removed via FLP-and Cre-mediated recombination in the germline. Western blot analysis revealed absence of expression of the long isoform in the brain (olfactory bulb, cerebral cortex, hippocampus, brain stem, cerebellum, and pituitary) and testis of homozygous mutant mice. In contrast, expression the canonical short isoform was upregulated in the olfactory bulb, cerebral cortex, hippocampus, brain stem, and cerebellum of homozygous mutant mice. (J:288930)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eef1d Mutation: 38 strains or lines available

References

• Original: J:288930 Kaitsuka T, et al., Deletion of Long Isoform of Eukaryotic Elongation Factor 1Bdelta Leads to Audiogenic Seizures and Aversive Stimulus-Induced Long-Lasting Activity Suppression in Mice. Front Mol Neurosci. 2018;11:358
• All: 1 reference(s)