Styk1^tm1Twa
Targeted Allele Detail

Summary

• Symbol: Styk1^tm1Twa
• Name: serine/threonine/tyrosine kinase 1; targeted mutation 1, Thierry Walzer
• MGI ID: MGI:6467474
• Synonyms: Styk1^EGFP
• Gene: Styk1 Location: Chr6:131276096-131330532 bp, - strand Genetic Position: Chr6, 63.44 cM
• Alliance: Styk1^tm1Twa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277150
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: EUCOMM vector PRPGS00082_B_F08 was modified to replace the lacZ gene with an EGFP cassette in the exon 2. It also contains the frt and LoxP sites that allow Cre-mediated conditional targeting of the gene following Flp-mediated recombination to remove the reporter gene. RT-QPCR analysis confirmed the absence of mRNA expression in NK cells from homozygous mutant mice. (J:277150)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Styk1 Mutation: 31 strains or lines available

References

• Original: J:277150 Fauteux S, et al., Styk1 expression is a hallmark of murine NK cells and other NK1.1(+) subsets but is dispensable for NK-cell development and effector functions. Eur J Immunol. 2019 May;49(5):677-685
• All: 1 reference(s)