Ighg1^{em2(IGHG1)Mvw}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ighg1^{em2(IGHG1)Mvw}
• Name: immunoglobulin heavy constant gamma 1 (G1m marker); endonuclease-mediated mutation 1, Michael V Wiles
• MGI ID: MGI:6467517
• Synonyms: hFC
• Gene: Ighg1 Location: Chr12:113290161-113294143 bp, - strand Genetic Position: Chr12, 62.1 cM
• Alliance: Ighg1^{em2(IGHG1)Mvw} page

Mutation origin

• Strain of Origin: B6.Cg-Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/DcrJ

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Ighg1^{em2(IGHG1)Mvw} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IGHG1 (3500)

  Ighg	(MGI:2144967)
  Ighg1	(MGI:96446)
  Ighg2a	(MGI:96443)
  Ighg2b	(MGI:96445)
  Ighg2c	(MGI:2686979)
  Ighg3	(MGI:2144790)

• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to replace the mouse Ighg1 locus hinge (exon 2), CH2 (exon 3) and CH3 (exon 4) regions with a 902 bp human hinge (exon 2), CH2 (exon 3) and CH3 (exon 4) sequences while preserving the splice junctions. (J:296090)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ighg1 Mutation: 28 strains or lines available

References

• Original: J:296090 Low BE, et al., Functional humanization of immunoglobulin heavy constant gamma 1 Fc domain human FCGRT transgenic mice. MAbs. 2020 Jan-Dec;12(1):1829334
• All: 1 reference(s)