Smoc2^{tm1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Smoc2^{tm1(cre/ERT2)Cle}
• Name: SPARC related modular calcium binding 2; targeted mutation 1, Hans Clevers
• MGI ID: MGI:6467827
• Synonyms: Smoc2-EGFP-ires-CreERT2
• Gene: Smoc2 Location: Chr17:14499768-14625052 bp, + strand Genetic Position: Chr17, 8.95 cM, cytoband A2
• Alliance: Smoc2^{tm1(cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186432
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Smoc2^{tm1(cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Smoc2 (MGI:1929881)

• Mutation details: An EGFP-IRES-creERT2 cassette was inserted at the translational start site of the gene along with a loxP-flanked neomycin cassette. The neomycin gene was then excised in vivo by crossing with a cre-deleter strain. EGFP expression is driven by the endogenous promoter. Cre expression is induced in Smoc2 expressing cells by tamoxifen treatment. (J:186432)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Smoc2 (mouse) Summary of all recombinase alleles driven by Smoc2. Comparative matrix view of recombinase activities Alliance mouse Smoc2 gene page

Expression

In Mice Carrying this Mutation:

8 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smoc2 Mutation: 23 strains or lines available

References

• Original: J:186432 Munoz J, et al., The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent '+4' cell markers. EMBO J. 2012;31(14):3079-91
• All: 2 reference(s)