Reln^em1Mhat
Endonuclease-mediated Allele Detail

Summary

• Symbol: Reln^em1Mhat
• Name: reelin; endonuclease-mediated mutation 1, Mitsuharu Hattori
• MGI ID: MGI:6468501
• Synonyms: PA-DV KI, Reln^PA-DV
• Gene: Reln Location: Chr5:22089452-22549700 bp, - strand Genetic Position: Chr5, 9.98 cM, cytoband A3-B1
• Alliance: Reln^em1Mhat page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated a CC to GA change resulting in a proline to aspartate substitution at amino acid 1244 (P1244D) as well as a C to T change resulting in an alanine to valine substitution at amino acid 1245 (A1245V). These mutations abolish the key cleavage site and make the protein uncleavable by ADAMTS-3. (J:293055)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Reln Mutation: 209 strains or lines available

References

• Original: J:293055 Okugawa E, et al., Physiological significance of proteolytic processing of Reelin revealed by cleavage-resistant Reelin knock-in mice. Sci Rep. 2020 Mar 11;10(1):4471
• All: 2 reference(s)