Gkap1^em1Feis
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gkap1^em1Feis
• Name: G kinase anchoring protein 1; endonuclease-mediated mutation 1, Fei Sun
• MGI ID: MGI:6470626
• Synonyms: GKAP1^-
• Gene: Gkap1 Location: Chr13:58381160-58422691 bp, - strand Genetic Position: Chr13, 30.97 cM, cytoband B2
• Alliance: Gkap1^em1Feis page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 2 was targeted with two sgRNAs using CRISPR/Cas9 technology, resulting in a 149 bp deletion. Western blot and immunofluorescence experiments confirmed virtual elimination protein expression from this allele. (J:293924)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gkap1 Mutation: 17 strains or lines available

References

• Original: J:293924 Wang H, et al., Functional role of GKAP1 in the regulation of male germ cell spontaneous apoptosis and sperm number. Mol Reprod Dev. 2019 Sep;86(9):1199-1209
• All: 1 reference(s)