Gt(ROSA)26Sor^{tm2(CAG-Ncre/vvd*,-vvd*/Ccre)Yzwa}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-Ncre/vvd*,-vvd*/Ccre)Yzwa}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Masayuki Yazawa
• MGI ID: MGI:6470712
• Synonyms: Gt(ROSA)26Sor^{tm2(CAG-cre*)Yzwa}, Rosa26^{PA-Cre B4}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-Ncre/vvd*,-vvd*/Ccre)Yzwa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292311
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase)
• Inducer: blue light exposure
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(CAG-Ncre/vvd*,-vvd*/Ccre)Yzwa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The targeting vector was designed with (from 5' to 3') a ubiquitously expressed CAG (CMV enhancer/chicken beta-actin) promoter, a frt-flanked STOP cassette (containing a neomycin resistance (neo) cassette and a polyadenylation [pA] signal). A PA-Cre 3.0 followed by a pA site was placed downstream of the frt-flanked STOP cassette. PA-Cre 3.0 consists of a split Cre (59/60) with each fragment complemented with nMag or pMag (respectively) and a nuclear localization sequence (NLS) (CreN59-nMag-NLS and NLS-pMag-CreC60), separated by P2A self-cleaving peptide sequence (2A). The nMag and pMag are part of Magnets protein derived from the fragments of photoreceptor gene product Vivid (VVD) from a filamentous fungi Neurospora crassa. This targeting vector was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into 129Sv/Ev x C57BL/6 derived embryonic stem (ES) cells (clone B4) and correctly targeted ES cells were injected into blastocysts. (J:292311)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:292311 Morikawa K, et al., Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun. 2020 May 1;11(1):2141
• All: 1 reference(s)