Pfkfb2^tm1.2Dap
Targeted Allele Detail

Summary

• Symbol: Pfkfb2^tm1.2Dap
• Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; targeted mutation 1.2, David A Power
• MGI ID: MGI:6470720
• Gene: Pfkfb2 Location: Chr1:130616919-130656990 bp, - strand Genetic Position: Chr1, 56.89 cM, cytoband E4
• Alliance: Pfkfb2^tm1.2Dap page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:296139
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The phospho-acceptor sites Ser⁴⁶⁸ and Ser⁴⁸⁵ located in exon 15 were mutated to alanine (S468A and S485A). A wild-type copy of exon 15 floxed by loxP sites was inserted into intron 14-15 upstream of the exon 15 splice acceptor followed by a polyadenylation signal. A FRT-flanked neomycin selection cassette was inserted into intron 14-15. FlpE-mediated recombination removed the neo cassette. Cre-mediated recombination removed the wild-type exon 15 so the mutant protein is expressed. (J:296139)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfkfb2 Mutation: 36 strains or lines available

References

• Original: J:296139 Lee M, et al., Mutation of regulatory phosphorylation sites in PFKFB2 worsens renal fibrosis. Sci Rep. 2020 Sep 3;10(1):14531
• All: 3 reference(s)