T^{em1.1(dre)Okn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: T^{em1.1(dre)Okn}
• Name: brachyury, T-box transcription factor T; endonuclease-mediated mutation 1.1, Hideyuki Okano
• MGI ID: MGI:6471923
• Synonyms: Brachyury^{2A-DredeltaPuro}
• Gene: T Location: Chr17:8653255-8661328 bp, + strand Genetic Position: Chr17, 4.92 cM
• Alliance: T^{em1.1(dre)Okn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284134
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NCrlCrlj

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• T^{em1.1(dre)Okn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	T (MGI:98472)

• Mutation details: CRISPR-targeting introduced at the end of the coding sequence in exon 8 a GSG (Gly-Ser-Gly)-linker, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, P1-like transducing phage D6 dre recombinase gene, and puromycin resistance cassette flanked by FRT sites (FRT/F3/F5). Flp-mediated recombination removed the selection cassette. (J:284134)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	T (mouse) Summary of all recombinase alleles driven by T. Alliance mouse T gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any T Mutation: 59 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

ES cells = B6NJ-22(UTR) (C57BL/6NCrlCrlj)

References

• Original: J:284134 Serizawa T, et al., Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Development. 2019 Nov 4;146(21):dev174938
• All: 1 reference(s)