Igfbp2^{em1(cre/ERT2)Ntang}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igfbp2^{em1(cre/ERT2)Ntang}
• Name: insulin-like growth factor binding protein 2; endonuclease-mediated mutation 1, Nan Tang
• MGI ID: MGI:6472828
• Synonyms: Igfbp2-CreER
• Gene: Igfbp2 Location: Chr1:72863662-72891633 bp, + strand Genetic Position: Chr1, 36.94 cM
• Alliance: Igfbp2^{em1(cre/ERT2)Ntang} page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Igfbp2^{em1(cre/ERT2)Ntang} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Igfbp2 (MGI:96437)

• Mutation details: The allele have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the insulin-like growth factor binding protein 2 locus (Igfbp2) on chromosome 1. The targeting vector contained, from 5' to 3', an IRES enhancer region, a CreER(T2) fusion gene (Cre-ER(T2); Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), and an SV40 polyadenylation sequence. The plasmid was targeted to the 3' UTR of the endogenous mouse Igfbp2 gene via CRISPR/Cas9 mediated gene targeting in C57BL/6N-derived zygotes. Offspring were bred with FVB mice for germline transmission of the allele. The resulting Igfbp2-CreER colony was then backcrossed to FVB mice for at least 6 generations prior to sending males. (J:260595)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Igfbp2 (mouse) Summary of all recombinase alleles driven by Igfbp2. Alliance mouse Igfbp2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igfbp2 Mutation: 23 strains or lines available

References

• Original: J:260595 Wang Y, et al., Pulmonary alveolar type I cell population consists of two distinct subtypes that differ in cell fate. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2407-2412
• All: 1 reference(s)