Prodh2^em1Jkni
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prodh2^em1Jkni
• Name: proline dehydrogenase (oxidase) 2; endonuclease-mediated mutation 1, John Knight
• MGI ID: MGI:6472936
• Gene: Prodh2 Location: Chr7:30193047-30212827 bp, + strand Genetic Position: Chr7, 18.36 cM, cytoband A3
• Alliance: Prodh2^em1Jkni page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated an 85 bp deletion in exon 2 resulting in a frameshift mutation. Western blot analysis confirmed the absence of full-length protein but presence of a truncated 380 bp form that is also seen in wild-type mice. (J:283585)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prodh2 Mutation: 14 strains or lines available

References

• Original: J:283585 Buchalski B, et al., The effects of the inactivation of Hydroxyproline dehydrogenase on urinary oxalate and glycolate excretion in mouse models of primary hyperoxaluria. Biochim Biophys Acta Mol Basis Dis. 2020 Mar 1;1866(3):165633
• All: 1 reference(s)