Rasgrf1^tm1bNarl
Targeted Allele Detail

Summary

• Symbol: Rasgrf1^tm1bNarl
• Name: RAS protein-specific guanine nucleotide-releasing factor 1; targeted mutation 1b, National Laboratory Animal Center
• MGI ID: MGI:6473478
• Gene: Rasgrf1 Location: Chr9:89791961-89909030 bp, + strand Genetic Position: Chr9, 47.31 cM
• Alliance: Rasgrf1^tm1bNarl page

Mutation origin

• Mutant Cell Line: WCW_1413_003
• Germline Transmission: Earliest citation of germline transmission: J:297104
• Parent Cell Line: WCW (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Pgk_P Vector Type: Not Applicable
• Mutation details: The targeting construct inserted upstream of exon 2 an FRT site, En2 splice acceptor, IRES sequence, lacZ reporter gene, SV40 polyadenylation sequence, loxP site, neomycin resistance cassette, and loxP site. An additional loxP site was inserted downstream of exon 2. Cre-mediated recombination removed exon 2 along with the reporter and selection cassettes. (J:297104)

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasgrf1 Mutation: 66 strains or lines available

References

• Original: J:297104 National Laboratory Animal Center, National Laboratory Animal Center Website. 2020
• All: 3 reference(s)