Zfyve27^tm1Kei
Targeted Allele Detail

Summary

• Symbol: Zfyve27^tm1Kei
• Name: zinc finger, FYVE domain containing 27; targeted mutation 1, Keiichi I Nakayama
• MGI ID: MGI:6473985
• Synonyms: protrudin-deficient, PRT^-
• Gene: Zfyve27 Location: Chr19:42159006-42183032 bp, + strand Genetic Position: Chr19, 35.87 cM
• Alliance: Zfyve27^tm1Kei page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303874
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.2-kbp fragment of genomic DNA containing exons I and II of the protrudin gene was replaced with a PGK-neo-poly(A)-loxP cassette. RT-PCR analysis of mRNA derived from regions corresponding to exon II or exon X demonstrated that the abundance of these transcripts was significantly reduced or nearly undetectable. (J:303874)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zfyve27 Mutation: 25 strains or lines available

References

• Original: J:303874 Shirane M, et al., Protrudin-deficient mice manifest depression-like behavior with abnormalities in activity, attention, and cued fear-conditioning. Mol Brain. 2020 Nov 10;13(1):146
• All: 2 reference(s)