Sinhcaf^tm1.2Hmd
Targeted Allele Detail

Summary

• Symbol: Sinhcaf^tm1.2Hmd
• Name: SIN3-HDAC complex associated factor; targeted mutation 1.2, Hiroshi Hamada
• MGI ID: MGI:6474233
• Synonyms: Fam60a^-
• Gene: Sinhcaf Location: Chr6:148822538-148847968 bp, - strand Genetic Position: Chr6, 78.67 cM, cytoband G3
• Alliance: Sinhcaf^tm1.2Hmd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313620
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted in intron 1 and an FRT-flanked IRES-Betageo and second loxP site were inserted in intron 4. Cre-mediated recombination removed exons 1-4 and the Betageo. RT-qPCR and Western blot analysis confirmed the absence of transcript and protein in embryos. (J:313620)

Expression

In Mice Carrying this Mutation:	11 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sinhcaf Mutation: 53 strains or lines available

References

• Original: J:313620 Nabeshima R, et al., Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters. Elife. 2018 Aug 2;7:e36435
• All: 2 reference(s)