Alk^{tm1.1(ALK*F1174L)Heno}
Targeted Allele Detail

Summary

• Symbol: Alk^{tm1.1(ALK*F1174L)Heno}
• Name: anaplastic lymphoma kinase; targeted mutation 1.1, Hideki Enomoto
• MGI ID: MGI:6476969
• Synonyms: Alk^flox
• Gene: Alk Location: Chr17:72175967-72911622 bp, - strand Genetic Position: Chr17, 43.77 cM
• Alliance: Alk^{tm1.1(ALK*F1174L)Heno} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294092
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Alk^{tm1.1(ALK*F1174L)Heno} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ALK (238)

  Alk	(MGI:103305)

• Mutation details: A gene cassette consisting of floxed wild-type human ALK cDNA-intron poly(A) followed by human ALK cDNA with a phenylalanine to leucine substitution at amino acid 1174 (F1174L), IRES, mitovenus cDNA-intron poly(A) and a neomycin resistance gene flanked by FRT sites was inserted into the first exon of the gene. The insertion of the gene cassette deletes 657 nucleotides in exon 1 of the gene (starting from -6 nucleotides upstream of the first ATG) and the deleted sequence encodes the first 217 amino acids including the signal sequence. The neo resistance gene was removed via Flp-mediated recombination. This allele expresses the human wild-type ALK by the endogenous Alk promoter. Cre-mediated recombination can be used to express the mutant protein. (J:294092)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Alk Mutation: 64 strains or lines available

References

• Original: J:294092 Ono S, et al., Generation of conditional ALK F1174L mutant mouse models for the study of neuroblastoma pathogenesis. Genesis. 2019 Oct;57(10):e23323
• All: 1 reference(s)