Gfi1^{tm2.1(GFP/cre/ERT2)Gan}
Targeted Allele Detail

Summary

• Symbol: Gfi1^{tm2.1(GFP/cre/ERT2)Gan}
• Name: growth factor independent 1 transcription repressor; targeted mutation 2.1, Lin Gan
• MGI ID: MGI:6477635
• Synonyms: Gfi1-GCE
• Gene: Gfi1 Location: Chr5:107864521-107873671 bp, - strand Genetic Position: Chr5, 52.23 cM
• Alliance: Gfi1^{tm2.1(GFP/cre/ERT2)Gan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:297267
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEv or C57BL/6J

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Gfi1^{tm2.1(GFP/cre/ERT2)Gan} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gfi1 (MGI:103170)

• Mutation details: The coding sequence for P2A-GFP/cre/ERT2 and a downstream FRT-flanked neomycin resistance cassette was inserted in-frame to the C-terminus resulting in bicistronic expression of the endogenous transcript and a tamoxifen-inducible cre recombinase with green fluorescent protein reporter. Breeding to a FLP deleter excised the neo and Immunolabelling of P0 cochlea shows normal nuclear localization of GFI1 and cytoplasmic expression of GFP/cre/ERT2, which migrates to the nucleus after tamoxifen administration. This allele expresses a high level of tamoxifen-inducible cre specific to developing hair cells of the vestibule and cochlea. (J:297267)
• Inheritance: Not Specified

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gfi1 (mouse) Summary of all recombinase alleles driven by Gfi1. Comparative matrix view of recombinase activities Alliance mouse Gfi1 gene page

Expression

In Mice Carrying this Mutation:

189 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gfi1 Mutation: 31 strains or lines available

References

• Original: J:297267 Tang Q, et al., Gfi1-GCE inducible Cre line for hair cell-specific gene manipulation in mouse inner ear. Genesis. 2019 Sep;57(9):e23304
• All: 1 reference(s)