Trp53^tm2Thst
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2Thst
• Name: transformation related protein 53; targeted mutation 2, Thorsten Stiewe
• MGI ID: MGI:6479073
• Synonyms: p53LSL-EE, p53LSL-R178E, Trp53^LSL-R178E
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2Thst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282255
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 5 was replaced with a modified one in which nucleotide substitutions CGC-to-GAG result in the amino acid substitution of arginine with glutamic acid at position 178 (p.R178E). A loxP site flanked STOP cassette was inserted into intron 1. (J:282255)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm2Thst

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:282255 Timofeev O, et al., Residual apoptotic activity of a tumorigenic p53 mutant improves cancer therapy responses. EMBO J. 2019 Oct 15;38(20):e102096
• All: 1 reference(s)