Ankmy2^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ankmy2^{tm1b(EUCOMM)Hmgu}
• Name: ankyrin repeat and MYND domain containing 2; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6488662
• Synonyms: Ankmy2 ko
• Gene: Ankmy2 Location: Chr12:36207123-36247290 bp, + strand Genetic Position: Chr12, 16.54 cM
• Alliance: Ankmy2^{tm1b(EUCOMM)Hmgu} page
• IMPC: Ankmy2 gene page

Mutation origin

• Mutant Cell Line: HEPD0679_6_C03
• Germline Transmission: Earliest citation of germline transmission: J:297055
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 36219981 of Chromosome 12 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 36220830. Exon 3 is thus flanked by loxP sites. Subsequent cre-mediated recombination removed the neo cassette and exon 3, creating a reporter knockout allele. Immunoblotting and qRT-PCR confirmed the lack of protein and transcript expression from this allele. (J:297055)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ankmy2 Mutation: 31 strains or lines available

References

• Original: J:297055 Somatilaka BN, et al., Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling. Dev Cell. 2020 Sep 28;54(6):710-726.e8
• All: 2 reference(s)