Carns1^tm1.2Meck
Targeted Allele Detail

Summary

• Symbol: Carns1^tm1.2Meck
• Name: carnosine synthase 1; targeted mutation 1.2, Matthias Eckhardt
• MGI ID: MGI:6489595
• Synonyms: Carns1^-
• Gene: Carns1 Location: Chr19:4214323-4225478 bp, - strand Genetic Position: Chr19, 3.86 cM
• Alliance: Carns1^tm1.2Meck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298850
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was introduced into intron 2 and a loxP and FRT site flanked neomycin resistance gene cassette downstream of exon 10. The neo cassette was removed through subsequent flp-mediated recombination; after that, exons 3-10 were deleted through cre-mediated recombination, leaving exons 1, 2 and the duplicated 3' end of exon 10. Northern and Western blot experiments confirmed the absence of mRNA and protein expression from this allele. (J:298850)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Carns1 Mutation: 35 strains or lines available

References

• Original: J:298850 Wang-Eckhardt L, et al., Carnosine synthase deficiency is compatible with normal skeletal muscle and olfactory function but causes reduced olfactory sensitivity in aging mice. J Biol Chem. 2020 Dec 11;295(50):17100-17113
• All: 1 reference(s)