St18^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: St18^{tm1c(KOMP)Wtsi}
• Name: suppression of tumorigenicity 18; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6489608
• Synonyms: St18^fl
• Gene: St18 Location: Chr1:6557455-6931164 bp, + strand Genetic Position: Chr1, 1.89 cM
• Alliance: St18^{tm1c(KOMP)Wtsi} page
• IMPC: St18 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298828
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 6808853 of Chromosome 1 into intron9 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted into intron 10 at position 6809630. Exon 10 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created through flp-mediated removal of the LacZ and neo cassettes. (J:298828)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any St18 Mutation: 71 strains or lines available

References

• Original: J:298828 Maruyama K, et al., Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages. Cell Rep. 2020 Jul 14;32(2):107906
• All: 1 reference(s)