Capn15^{tm2d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Capn15^{tm2d(EUCOMM)Hmgu}
• Name: calpain 15; targeted mutation 2d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6489917
• Gene: Capn15 Location: Chr17:26177338-26204753 bp, - strand Genetic Position: Chr17, 13.04 cM, cytoband A3
• Alliance: Capn15^{tm2d(EUCOMM)Hmgu} page

Mutation origin

• Mutant Cell Line: HEPD0809_1_C08
• Germline Transmission: Earliest citation of germline transmission: J:298296
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26181430 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 26182779. Flp-mediated recombination removed the FRT-flanked lacZ/neo cassette. Cre-mediated recombination removed exons 3-5. (J:298296)

Expression

In Mice Carrying this Mutation:	15 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Capn15 Mutation: 30 strains or lines available

References

• Original: J:298296 Zha C, et al., Biallelic variants in the small optic lobe calpain CAPN15 are associated with congenital eye anomalies, deafness and other neurodevelopmental deficits. Hum Mol Genet. 2020 Nov 4;29(18):3054-3063
• All: 2 reference(s)