Sh3yl1^tm1Syb
Targeted Allele Detail

Summary

• Symbol: Sh3yl1^tm1Syb
• Name: Sh3 domain YSC-like 1; targeted mutation 1, Yun Soo Bae
• MGI ID: MGI:6489952
• Synonyms: SH3YL1 KO
• Gene: Sh3yl1 Location: Chr12:30961667-31010161 bp, + strand Genetic Position: Chr12, 13.01 cM
• Alliance: Sh3yl1^tm1Syb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298830
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector used to create the Sh3yl1^{tm1a(KOMP)Wtsi} allele was used to create this knockout allele. The L1L2_Bact_P cassette was inserted at position 30939123 of Chromosome 12 upstream of exon 5 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 30939920. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:298830)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sh3yl1 Mutation: 19 strains or lines available

References

• Original: J:298830 Yoo JY, et al., LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1. Cell Rep. 2020 Oct 20;33(3):108245
• All: 2 reference(s)