Pole3^em2Tbo
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pole3^em2Tbo
• Name: polymerase (DNA directed), epsilon 3 (p17 subunit); endonuclease-mediated mutation 2, Thomas Boehm
• MGI ID: MGI:6490634
• Synonyms: Pole3^m2
• Gene: Pole3 Location: Chr4:62440889-62443305 bp, - strand Genetic Position: Chr4, 33.17 cM, cytoband C1
• Alliance: Pole3^em2Tbo page

Mutation origin

• Strain of Origin: FVB

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Intragenic deletion
• Mutation details: A 131 bp deletion in the 3' end of the coding region was created using sgRNAs (targeting TACAGGCGGGAGCAGAA and ACGACCAGAACGAAGAGG) with CRISPR/Cas9 technology. The deletion removes most of the negatively charged tail of the encoded peptide. (J:298827)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pole3 Mutation: 14 strains or lines available

References

• Original: J:298827 Siamishi I, et al., Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. Cell Rep. 2020 Jun 16;31(11):107756
• All: 1 reference(s)