Adgrl2^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Adgrl2^{tm1b(EUCOMM)Hmgu}
• Name: adhesion G protein-coupled receptor L2; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6491650
• Gene: Adgrl2 Location: Chr3:148521219-148696191 bp, - strand Genetic Position: Chr3, 74.1 cM
• Alliance: Adgrl2^{tm1b(EUCOMM)Hmgu} page
• IMPC: Adgrl2 gene page

Mutation origin

• Mutant Cell Line: HEPD0631_8_B02
• Germline Transmission: Earliest citation of germline transmission: J:297371
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 148570652 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 148571811. The critical exon(s) is/are thus flanked by loxP sites. Cre expression without flp expression produced a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:297371)

Expression

In Mice Carrying this Mutation:	16 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adgrl2 Mutation: 83 strains or lines available

References

• Original: J:297371 Lee CS, et al., Identification of Latrophilin-2 as a Novel Cell-Surface Marker for the Cardiomyogenic Lineage and Its Functional Significance in Heart Development. Circulation. 2019 Jun 18;139(25):2910-2912
• All: 1 reference(s)