Tmem120a^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem120a^{tm1c(EUCOMM)Wtsi}
• Name: transmembrane protein 120A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6492190
• Synonyms: TACAN^fl
• Gene: Tmem120a Location: Chr5:135764344-135773026 bp, - strand Genetic Position: Chr5, 75.38 cM
• Alliance: Tmem120a^{tm1c(EUCOMM)Wtsi} page
• IMPC: Tmem120a gene page

Mutation origin

• Mutant Cell Line: EPD0327_2_H11
• Germline Transmission: Earliest citation of germline transmission: J:287046
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 135770595 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 135771713. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287046)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem120a Mutation: 21 strains or lines available

References

• Original: J:287046 Beaulieu-Laroche L, et al., TACAN Is an Ion Channel Involved in Sensing Mechanical Pain. Cell. 2020 Mar 5;180(5):956-967.e17
• All: 1 reference(s)