Nup210^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Nup210^{tm1b(EUCOMM)Hmgu}
• Name: nucleoporin 210; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6501962
• Synonyms: Nup210 KO
• Gene: Nup210 Location: Chr6:90990050-91093811 bp, - strand Genetic Position: Chr6, 40.3 cM
• Alliance: Nup210^{tm1b(EUCOMM)Hmgu} page
• IMPC: Nup210 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266693
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91066582 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 91067395. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:266693)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nup210 Mutation: 85 strains or lines available

References

• Original: J:266693 van Nieuwenhuijze A, et al., Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol. 2018;9:2234
• All: 1 reference(s)