Med23^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Med23^{tm1c(KOMP)Wtsi}
• Name: mediator complex subunit 23; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6502590
• Synonyms: Med23^flox
• Gene: Med23 Location: Chr10:24745889-24789358 bp, + strand Genetic Position: Chr10, Syntenic
• Alliance: Med23^{tm1c(KOMP)Wtsi} page
• IMPC: Med23 gene page

Mutation origin

• Mutant Cell Lines: EPD0332_6_B08, EPD0332_6_D07
• Germline Transmission: Earliest citation of germline transmission: J:299019
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 24766526 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 24768823. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:299019)

Expression

In Structures Affected by this Mutation:

18 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med23 Mutation: 75 strains or lines available

References

• Original: J:299019 Dash S, et al., The Mediator Subunit, Med23 Is Required for Embryonic Survival and Regulation of Canonical WNT Signaling During Cranial Ganglia Development. Front Physiol. 2020;11:531933
• All: 2 reference(s)