Map9^tm1.2Bcgen
Targeted Allele Detail

Summary

• Symbol: Map9^tm1.2Bcgen
• Name: microtubule-associated protein 9; targeted mutation 1.2, Biocytogen LLC
• MGI ID: MGI:6502642
• Synonyms: Map9^delta
• Gene: Map9 Location: Chr3:82265379-82302575 bp, + strand Genetic Position: Chr3, 36.37 cM
• Alliance: Map9^tm1.2Bcgen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:299895
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A gene trap cassette, consisting of an FRT site, an engrailed 2 splice acceptor site, IRES, EGFP, a loxP site, a neomycin selection gene, another FRT site and a loxP flanked exon 5, was inserted into intron 4. Flp-mediated recombination removed the EGFP and neomycin gene, leaving exon 5 floxed. Exon 5 was removed via cre-mediated recombination in the germline resulting in a frameshift and premature stop codon in exon 6. qPCR confirmed the absence of mRNA in homozygotes. (J:299895)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map9 Mutation: 27 strains or lines available

References

• Original: J:299895 Wang S, et al., MAP9 Loss Triggers Chromosomal Instability, Initiates Colorectal Tumorigenesis, and Is Associated with Poor Survival of Patients with Colorectal Cancer. Clin Cancer Res. 2020 Feb 1;26(3):746-757
• All: 1 reference(s)