Rxfp1^em1(cre)Ngai
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rxfp1^em1(cre)Ngai
• Name: relaxin/insulin-like family peptide receptor 1; endonuclease-mediated mutation 1, John Ngai
• MGI ID: MGI:6502651
• Synonyms: Rxfp1-P2A-Cre
• Gene: Rxfp1 Location: Chr3:79548918-79645187 bp, - strand Genetic Position: Chr3, 34.94 cM
• Alliance: Rxfp1^em1(cre)Ngai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300311
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Rxfp1^em1(cre)Ngai expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rxfp1 (MGI:2682211)

• Mutation details: The bicistronic Rxfp1-P2A-Cre knock-in allele was created using homologous recombination (aided by targeted CRISPR/cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (cre) the gene. The targeting vector, Cas9 endonuclease, and guide-RNA were mixed and injected into the v6.5 mouse embryonic stem cells. Colonies grown from transfected cells were directly screened for successful integration and were aggregated with albino morula and implanted into pseudopregnant females, which produced germ-line-competent chimeric founders. The ampicillin cassette is still present in this allele. (J:300311)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rxfp1 (mouse) Summary of all recombinase alleles driven by Rxfp1. Comparative matrix view of recombinase activities Alliance mouse Rxfp1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rxfp1 Mutation: 53 strains or lines available

References

• Original: J:300311 Pool AH, et al., The cellular basis of distinct thirst modalities. Nature. 2020 Dec;588(7836):112-117
• All: 1 reference(s)