Med19^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Med19^{tm1c(EUCOMM)Wtsi}
• Name: mediator complex subunit 19; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6502866
• Synonyms: MED19^Lox
• Gene: Med19 Location: Chr2:84508746-84518559 bp, + strand Genetic Position: Chr2, 49.45 cM, cytoband E1
• Alliance: Med19^{tm1c(EUCOMM)Wtsi} page
• IMPC: Med19 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298822
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 84515114 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 84516884. Exons 2-4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:298822)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med19 Mutation: 15 strains or lines available

References

• Original: J:298822 Dean JM, et al., MED19 Regulates Adipogenesis and Maintenance of White Adipose Tissue Mass by Mediating PPARgamma-Dependent Gene Expression. Cell Rep. 2020 Oct 6;33(1):108228
• All: 1 reference(s)