Kctd1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kctd1^{tm1c(EUCOMM)Wtsi}
• Name: potassium channel tetramerisation domain containing 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6502868
• Synonyms: Kctd1^fl, KCTD1^fl
• Gene: Kctd1 Location: Chr18:15101742-15284503 bp, - strand Genetic Position: Chr18, 8.28 cM
• Alliance: Kctd1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Kctd1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:297058
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 15119025 of Chromosome 18 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 15120110. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:297058)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kctd1 Mutation: 39 strains or lines available

References

• Original: J:297058 Marneros AG, AP-2beta/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis. Dev Cell. 2020 Aug 10;54(3):348-366.e5
• All: 2 reference(s)