Kctd1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kctd1^{tm1d(EUCOMM)Wtsi}
• Name: potassium channel tetramerisation domain containing 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6502870
• Synonyms: Kctd1^-, KCTD1 KO
• Gene: Kctd1 Location: Chr18:15101742-15284503 bp, - strand Genetic Position: Chr18, 8.28 cM
• Alliance: Kctd1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Kctd1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:297058
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 15119025 of Chromosome 18 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 15120110. Exon 3 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:297058)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kctd1 Mutation: 39 strains or lines available

References

• Original: J:297058 Marneros AG, AP-2beta/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis. Dev Cell. 2020 Aug 10;54(3):348-366.e5
• All: 3 reference(s)