Med22^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Med22^{tm1c(EUCOMM)Hmgu}
• Name: mediator complex subunit 22; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6503811
• Synonyms: Med22-fl
• Gene: Med22 Location: Chr2:26795279-26800654 bp, - strand Genetic Position: Chr2, 19.09 cM
• Alliance: Med22^{tm1c(EUCOMM)Hmgu} page
• IMPC: Med22 gene page

Mutation origin

• Mutant Cell Line: HEPD0884_4_E12
• Germline Transmission: Earliest citation of germline transmission: J:300239
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26797837 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 26798761. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:300239)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med22 Mutation: 13 strains or lines available

References

• Original: J:300239 Rodriguez PQ, et al., Inactivation of mediator complex protein 22 in podocytes results in intracellular vacuole formation, podocyte loss and premature death. Sci Rep. 2020 Nov 18;10(1):20037
• All: 1 reference(s)